Evidence for aWolbachiasymbiont inDrosophila melanogaster

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Abstract
Summary: The bacterial cell division gene,ftsZ, was used as a specific probe to show the presence of a symbiotic bacterium in two wild type strains ofDrosophila melanogaster. Under stringent hybridization conditions we have shown that the bacterium is transferred to the progeny of these strains from infected mothers and can be eradicated by treatment with the antibiotic tetracycline. We have characterized this bacterium, by amplifying and sequencing its 16S rRNA gene, as being a member of the genus Wolbachia, an organism that is known to parasitize a range of insects includingDrosophila simulans. In a series of reciprocal crosses no evidence was found that the symbiont causes cytoplasmic incompatibility (CI) which is known to occur in infected strains ofD. simulans. The implications of these findings are discussed.