Specific capture of ABH blood group antigens of the red cell or body fluids by double antibody sandwich-ELISA.
- 1 January 1989
- journal article
- research article
- Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine
- Vol. 158 (3) , 211-219
- https://doi.org/10.1620/tjem.158.211
Abstract
A double antibody sandwich-ELISA method for the detection of the ABH blood group of each constituent of mixed stains is described. Extracts from mixed stains were applied to microtitration plates coated with rabbit polyclonal antisera to red cells or body fluids. ABH antigens in body fluid stains which were captured by the polyclonal antibodies were detected by monoclonal anti-A and -B and enzyme-conjugated anti-mouse immunoglobulin. By this procedure, ABH antigens of only saliva, semen or red cells could be detected from mixed stains, but no ABH antigen capture activity was observed using anti-sweat, -milk, -vaginal secretion, -erythrocyte membrane and -band 3 antibodies.Keywords
This publication has 0 references indexed in Scilit: