Light and Scanning Electron Microscopy of Fluorosed Enamel from Human Permanent Teeth

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Abstract
Acid-etched, ground surfaces of the enamel of 9 fluorosed teeth were studied with scanning electron microscopy, and ground sections from adjacent parts of the same teeth were studied with light microscopy and microradiography. 4 non-fluorosed, intact teeth served as control material. The scanning technique proved to be a sensitive technique for demonstrating slight changes in fluorosed enamel. In comparison with normal enamel, fluorosed enamel showed an overall accentuation of Retzius lines and intraprismatic cross-striations. The outer part of fluorosed enamel often showed a surface zone with a coarsely granular texture in which the prismatic structure was barely detectable, and an underlying subsurface part with a hollowed-out appearance. These findings were discussed in relation to in vivo conditions and the pathogenesis of enamel fluorosis.

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