Studies on Platelet Factor 3 Activity

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Abstract
A material consisting of 13 normal controls and 32 patients suffering from various types of secondary thrombocytopathy and some special cases has been tested by a modified thrombin generation test and Stypven clotting time in order to evaluate platelet factor 3 activity.Intact platelet suspensions, platelets damaged by freezing and thawing or ultrasonic vibrations and phospholipid extracts have been added to platelet‐free, non‐activated citrated plasma prepared from the same normal person in all experiments. The effect on velocity and amount of thrombinformation and on Stypven clotting time have been estimated in the total group. The results have been evaluated statistically.The modified thrombin generation test did not show any significant differences in activity in any cases tested.The Stypven‐test was technically much more satisfactory and significantly less activity was found using platelets from patients with hepatic cirrhosis. The concentration of phosphatidylserine was normal in these platelets.In vitro experiments with surface‐active agents support the hypothesis, that surface charge of phosphatide micelles are of decisive importance for their coagulation accelerating effect.