Abstract
19 strains of lactobacilli, including human dental and intestinal forms and strains from normal rat mouths, were tested for acid production limits in a 2% glucose, dilute (1-6) veal broth, titrated to pH 2.8-6.6, and for acid tolerance in a stock veal broth titrated to pH 2.2-4.8. All pH determinations were made with a glass electrode-vacuum tube potentiometer. Results indicate that the most strongly acidogenic lactobacilli, including members from dental and intestinal groups, are able to attain a pH slightly above 3 as the initial pH of the medium is progressively reduced; the weakest strains, including chiefly rat forms, do not reduce the pH of the medium below 4. and are in this respect similar to Bacterium coli and Staphylococcus aureus. A correlation between growth strength and acid production is indicated. Tests for acid-tolerance limits, thus far incomplete, indicate that this property parallels acid production for a given strain.

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