Oxidative Metabolism Associated with Phagocytosis in Acanthamoeba castellanii1

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Abstract
When amebae were incubated with latex beads, cyanide‐insensitive oxygen consumption increased nearly two‐fold. This cyanide‐insensitive respiration was inhibited by salicylhydroxamate. Furthermore, cell fractionation studies revealed a localization for a portion of the NAD(P)H oxidase activity in phagolysosomes. The presence of low concentrations of divalent metal during fractionation resulted in an increased yield of oxidative activity in the phagolysosome fraction. In addition, the phagolysosome membrane was enriched about two‐fold in a b‐type cytochrome. These results show that oxidative metabolism in amebae has some striking similarities to the respiratory burst oxidase of neutrophils.