Abstract
Concentration of hydroxyproline, as a measure of collagen, has been determined in liver, gastric tissue, skin and bone of male rats. Normal values of control, untreated, rats were compared with collagen values of the tissues in animals, submitted to prolonged treatment with cortisone. Highly significant increase of liver, stomach and bone collagen was found in cortisone treated rats. Hydroxyproline of rats'' skin was not altered by cortisone.

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