Effect of Oxidative Phosphorylation Inhibitors on Synthesis of Liver Mitochondria Phospholipids.

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Abstract
The effect of single dose of arsenate (14 mg/kg), bilirubin (3.3 mg/kg), oligomycin (0.83 mg/kg) and dinitro-phenol (30 mg/kg) on the synthesis of mitochondria phospholipids of the liver of male albino rats (Sprague-Dawley strain, 100-140 g) was studied. Intraperitoneal injection of 200 [mu]c of P32 as NaH2PO4 was administered one hour following injection of above compounds. Four hours later the animals killed, liver removed, mitochondria prepared, lipids extracted and chromatographed on silicic acid impregnated glass filter paper, radioactivity and phosphorus determined on the individual phospholipid fractions. A statistically significant decrease occurred in synthesis of phosphatidyl inositol, sphingomyelin, lecithin, phosphatidyl serine and phosphatidyl ethanolamine of liver mitochondria following administration of dinitrophenol. Administration of arsenate, oligomycin and bilirubin inhibited statistically the incorporation of the isotope into all the phospholipid fractions of liver mitochondria except sphingomyelin and phosphatidyl inositol. However, oligomycin inhibited incorporation of the isotope into sphingomyelin fraction and arsenate inhibited the phosphatidyl inositol fraction.