Comparison of the Responsiveness of Perirenal Adipose Tissue of the Rat, Hamster, Guinea Pig and Rabbit to the Antilipolytic Action of Insulin

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Abstract
Previous studies in several laboratories have demonstrated marked species differences in the hormonal responsiveness of mammalian adipose tissue. The present investigation extends these comparative studies by comparing the antilipolytic potency of insulin upon the perirenal adipose tissue of rat, hamster, guinea pig and rabbit. Epididymal adipose tissue of the rat was also studied. Lipolysis was stimulated in vitro in slices of adipose tissue from these 4 species by incubation with 1 [mu]g/ml epinephrine, adrenocorticotropin (ACTH) or [beta]-melanocyte-stimulating hormone ([beta]-MSH) in albumin-free, glucose-free Krebs-Ringer phosphate medium; the lipolytic response to these hormones, in the absence and presence of .00001-10 [mu]g/ml insulin, was determined by measuring the free fatty acid (FFA) concentration in the tissue slices after a 2-hr, incubation. Bovine insulin at a concentration of .0001-. 001 [mu]g/ml or greater inhibited the lipolytic response of rat adipose tissue to epinephrine or ACTH by 50-95%. At a concentration of .01 [mu]g/ml or more, bovine insulin exerted a similar antilipolytic action on hamster adipose tissue slices stimulated by epinephrine or ACTH. In contrast, bovine, ovine or porcine insulins at a concentration of 10 [mu]g/ml failed to influence the lipolytic response of guinea pig adipose tissue to ACTH, or of rabbit adipose tissue to ACTH or [beta]-MSH. Insulin prepared from rabbit pancreas did not exert an antilipolytic action on rabbit adipose tissue at a concentration of 1 [mu]g/ml, although as little as . 01 [mu]g/ml of this insulin preparation exhibited pronounced antilipolytic effect upon the adipose tissue of the rat and hamster.

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