METABOLISM OF 7,12-DIMETHYLBENZ[A]ANTHRACENE IN MOUSE SKIN HOMOGENATES ANALYZED WITH HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

  • 1 January 1977
    • journal article
    • research article
    • Vol. 5  (3) , 295-301
Abstract
The metabolism of the carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) in epidermal homogenates from 3-methylcholanthrene[MC]-pretreated mice was analyzed with high pressure liquid chromatography. Metabolism was undetectable in the absence of pretreatment. Specific activities in epidermal homogenates from pretreated mice were approximately 100-1000 times lower than those observed in comparable incubations containing hepatic microsomes from MC-pretreated rats. The major metabolite formed was identified as 7-hydroxymethyl-12-methylbenz[a]anthracene. Each of the known hydroxymethyl metabolites was also present in detectable quantities. The K-region diol was not measurably present in incubations with mouse skin homogenates or rat liver microsomes from MC-pretreated animals. 7,8-Benzoflavone, 5,6-benzoflavone and 17-.beta.-estradiol were potent inhibitors of the metabolic transformation of DMBA by epidermal homogenates in vitro, whereas butylated hydroxytoluene and 1,1,1,-trichloro-2,3-propene oxide had little effect on or enhanced metabolite formation from DMBA in vitro.

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