Fluorometric high performance liquid chromatographic analysis of prostaglandin(PG) metabolites: Application to the catabolism of PGE2 and PGF2.ALPHA. in rat and rabbit kidney homogenates.

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Abstract
A previously reported fluorometric high performance liquid chromatographic procedure for separation of 9-anthryldiazomethane (ADAM) derivatives of prostaglandins (PG''s) was applied to examine catabolic products of PG''s, such as 15-keto-PGE2, 13,14-dihydro-15-keto-PGE2, 15-keto-PGF2.alpha., and 13,14-dihydro-15-keto-PGF2.alpha.. ADAM derivatives of these metabolites were separated in 30 min on an ODS column with 65% acetonitrile. By this method catabolism of PGE2 and PGF2.alpha. in kidney homogenates of rats and rabbits was analyzed. Homogenates of medulla and cortex of rat kidney catabolized PGE2 and PGF2.alpha. to 15-keto-PG''s. Whereas the homogenate of rabbit kidney cortex yielded 13,14-dihydro-15-keto-PGE2, that of medulla did not. The conversion of PGF2.alpha. to PGE2 was shown to occur in homogenates of rabbit kidney cortex as well as rat kidney medulla and cortex.

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