Long-term Cultivation of Pseudo-diploid Cells of Human Tumor Origin

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Abstract
One cell culture line was obtained from ascites fluid of a case diagnosed as reticulosarcomatosis. After about 50 days lag period, a cell line which could grow continuously in vitro was established. This cell line has grown only in a floating state. At the present time, the doubling time of the culture is within 30 hours and the cells multiply actively. Chromosomal analysis showed that the model number of this line was 46, but the karyotype was not the same as that of the normal human cells. So far this pseudo-diploidal character has not changed in its successive culture.