INHIBITION OF RIBONUCLEOSIDE METABOLISM IN EHRLICH ASCITES TUMOR CELLS BY PURINE ANALOGUE RIBONUCLEOSIDES
- 1 October 1965
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 43 (10) , 1701-1710
- https://doi.org/10.1139/o65-188
Abstract
Several aspects of the metabolism of inosine and uridine by Ehrlich ascites carcinoma cells in-vitro have been found to be inhibited by ribonucleoside derivatives of 4 purine analogues. The synthesis of both inosine and uridine by intact tumor cells was profoundly inhibited in the presence of 6-methylmercaptopurme ribonucleoside. Also inhibited were inosine and uridine cleavage, and the exchange of isotope between these ribonucleosides and the corresponding C14 -labelled bases. These reactions, however, were not inhibited when they took place in broken-cell preparations. Similarly, inosine metabolism in intact cells (but not in broken cells) was profoundly inhibited by 3 related compounds: the ribonucleosides of the 6-chloro. 6-methylmercapto, and 6-propylmercapto derivatives of 2-aminopurine.This publication has 1 reference indexed in Scilit:
- Synthesis of 5-Phosphoribosyl 1-Pyrophosphate from Ribonucleosides in Ehrlich Ascites Tumor Cells in VitroJournal of Biological Chemistry, 1965