Polymerase Chain Reaction-Based DNA Fingerprinting for Identification of Streptococcus and Enterococcus Species Isolated from Bovine Milk
- 1 March 1994
- journal article
- Published by Elsevier in Journal of Food Protection
- Vol. 57 (3) , 240-245
- https://doi.org/10.4315/0362-028x-57.3.240
Abstract
A total of 353 isolates including 304 isolates from bovine milk belonging to 8 Streptococcus and Enterococcus species, 14 American Type Culture Collection reference strains, 10 isolates from bovine milk that had atypical biochemical profiles, and 25 bovine milk isolates from Vermont were examined by polymerase chain reaction-based DNA fingerprinting. A single short oligonucleotide primer of eight bases (5′-GTAACGCC-3′) was used to produce a characteristic spectrum of amplified DNA fragments. Amplified DNA fragments were visualized following agarose gel electrophoresis and analyzed by scanning laser densitometry. Amplified DNA fragments were categorized as either primary, secondary, or variable fragments. DNA fragments observed in all isolates within a species were designated as primary [optical density > 0.3 absorbance units (AU)] and secondary fragments (optical density > 0.15 AU), while infrequent DNA fragments were designated as variable fragments (optical density >0.15 AU). Examination of fingerprint ...Keywords
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