Structural and enzymological analysis of the interaction of isolated domains of cytochrome P‐450 BM3
- 18 April 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 343 (1) , 70-74
- https://doi.org/10.1016/0014-5793(94)80609-8
Abstract
The interactions of the individually expressed haem‐ and flavin‐containing domains of cytochrome P‐450 BM3 have been analysed by enzymological and spectroscopic techniques. Electron transfer between the isolated domains occurs at a much lower rate than that occurring in the intact flavocytochrome. CD spectroscopic studies indicate that the linkage of the domains in intact P‐450 BM3 creates haem and amino acid environments suitable for efficient electron transfer from its flavin domain.Keywords
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