A quick screening test of biomaterials by means of chick embryo techniques

Publisher Website
Google Scholar
Abstract
Many types of materials are being used as biomaterials, and there is a continuing search for more and better materials for specific pur poses. In this investigation, a new method was employed in screening materials in an effort to cut costs, save time, and have a worthwhile indication of the prospect of a new material. Samples of twenty gauge wires of stainless steel 316, stainless steel 304, Titanium 40, Vitallium, and copper were introduced into leg or wing muscles of 18‐day‐old chick embryos.This was accomplished by opening the egg shell with a dental drill, implanting the material, and reclosing the shell. The chicks were allowed to hatch and were later sacrificed after eleven days, giving a total implant time of 14 days. This time period allowed for the acute inflammation that occurred during wound healing to subside. The sections were retrieved, embedded in epon 812, sectioned with a diamond saw, and histologically evaluated.Copper was used for the negative control and showed the greatest adverse tissue response. Titanium 40 showed almost no response and stainless steel 316 showed very little. Stainless steel 304 exhibited a somewhat greater response than the stainless steel 316. All of the materials except wrought Vitallium used in this experiment gave predicted results.

This publication has 8 references indexed in Scilit: