Inactivation of cryptosporidium at 1°c using ozone or chlorine dioxide

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Abstract
Inactivation of cesium chloride purified, bovine‐derived C. parvum oocysts was studied at bench‐scale using ozone or chlorine dioxide at 1°C. Experiments were conducted in 200 mL of 0.05 M phosphate buffer using a temperature controlled water bath. Animal infectivity using neonatal CD‐1 mice was used for evaluation of oocyst viability after treatment. The results showed that while the parasite could be killed at 1°C, the level of effort was substantially higher than that found at 22°C. An ozone Ct product (final residual and contact time product) of 7.2 to 15 mg‐min./L at pH 6.9 and 1°C resulted in an inactivation of 0.7 to 1.3 log‐units. This was about one‐third to one‐fifth of that observed at room temperature using a similar Ct product. A chlorine dioxide Ct product of 120 to 135 mg•min./L at pH 6.0 and 1°C resulted in an inactivation of 0.5 to 0.6 log‐units. This was about one‐fourth the kill observed at 22°C using a similar Ct product.

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