In Vitro Synthesis of Cowpea Chlorotic Mottle Virus Polypeptides
- 1 August 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 44 (2) , 545-549
- https://doi.org/10.1099/0022-1317-44-2-545
Abstract
The RNA of cowpea chlorotic mottle virus were separated by rate zonal centrifugation, followed by polyacrylamide gel electrophoresis. They were then translated in wheat germ extracts and rabbit reticulocyte lysates. RNA 4 codes for a polypeptide of about 19,000 and RNA 3 is translated into a polypeptide of 32,000 daltons. RNA 2 and 1 each code for polypeptides of about 105,000 daltons. Several smaller polypeptides were also synthesized and these are probably incomplete products. Of the 2 minor RNA components (RNA 5 and 6) found in isolated virions, only RNA 5 could be translated, the major product being about 68,000 daltons.This publication has 1 reference indexed in Scilit:
- Synthesis of Proteins in Tobacco Protoplasts Infected with Cowpea Chlorotic Mottle VirusJournal of General Virology, 1977