Insertion of transposons through the major cotransduction gap of Escherichia coli K-12

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Abstract
The major cotransduction gap of the E. coli chromosome extends from min 31 to 34. Transposons were inserted through this gap which, by sequential transductions, link sbcA (min 29.8) with manA (min 35.7) and thus eliminate the gap. The length of DNA in the region, as measured by transduction, is not significantly different from the length obtained by conjugational time of entry. Since this segment of the E. coli chromosome has few known genes, these transposon insertions will be useful for genetic manipulations in the region of the gap. The usefulness of these markers for rapidly mapping mutations which may be isolated in the region from min 27 to 37 were described.