A rapid and sensitive liquid chromatography/tandem mass spectrometry method for determination of docetaxel in human plasma

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Abstract
A novel, rapid and sensitive isocratic liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for quantification of docetaxel in human plasma with paclitaxel as internal standard. The high sensitivity and specificity of MS/MS detection enabled the use of a small volume of plasma (0.05 mL) and a simple liquid‐liquid extraction procedure. Furthermore, a very short run‐time (3 min) fulfilled the need for monitoring plasma levels of docetaxel from large‐scale clinical studies. The calibration curve for docetaxel was linear over the range 5–1000 ng/mL with coefficients of correlation >0.999 using only 0.05 mL plasma. The intra‐ and inter‐day precisions (CV) of analysis were 2. Possible conjugated metabolites of docetaxel were not detected in patients' samples. Copyright © 2003 John Wiley & Sons, Ltd.

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