Enterobacter pulveris sp. nov., isolated from fruit powder, infant formula and an infant formula production environment

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Abstract
Six Gram-negative, facultative anaerobic, non-spore-forming isolates of coccoid rods were obtained from fruit powder (n=3), infant formula (n=2) and infant formula production environment (n=1) and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB sequence analysis, allocated the isolates to the Enterobacteriaceae. The highest rpoB sequence similarities (91.2-95.8 %) were obtained with Enterobacter helveticus, Enterobacter radicincitans, Enterobacter turicensis and Enterobacter sakazakii and the phylogenetic branch formed by these species is supported by a high bootstrap value. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by the positive utilization of α-D-melibiose, sucrose, D-arabitol, mucate and 1-0-methyl-α-galacto-pyranoside as well as negative tests for D-sorbitol, and the Voges-Proskauer reaction. ud On the basis of the phylogenetic analyses, DNA-DNA hybridizations and the unique physiological and biochemical characteristics, it is proposed that the isolates represent a novel Enterobacter species, Enterobacter pulveris sp. nov. The type strain is 601/05T (= LMG 24057T = DSM 19144T)

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