The Effect of Nonanal on Dipodascus aggregatus II. Influence of the Prehistory of the Inoculum and of the Presence of Ethanol

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Abstract
Nonanal, added in ethannlic solution, in concentrations lower than 40 to 80 μM did not affect the growth of Dipodascus aggregatus, provided the inoculum had been harvested from the exponential phase of growth. Growth could even be inhibited by 80 μM.If the inoculum had been grown to the exponential phase and then for another period, to the acceleration phase, in fresh liquid medium, growth was strongly promoted by 80 μM nonanal. If cells from the exponential phase were grown for another period in the supernatant fluid of centrifuged cultures from the exponential phase, 80 μM affected growth in the following way: in five different experiments growth was not stimulated, in one experiment undoubtedly promoted, and weakly stimulated in another one.The growth of cultures inoculated with cells grown only on malt agar was not affected by 80 μM nonanal.Pretreatment of cells, harvested from the acceleration phase, with nonanal (80 μM) in the presence of ethanol did not diminish the growth‐promoting action of nonanal on the cultures inoculated with these cells. Nonanal, in the absence of ethanol, in a concentration of 10 μM did not affect the growth of cells, harvested from the acceleration phase, whereas 100 μM nonanal strongly inhibited growth.An attempt is made to explain the results starting from the endogenous metabolism.