The Sorption Behavior of Alkyl Bonded Phases in Reverse-Phase, High Performance Liquid Chromatography

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Abstract
A comparison of selectivity data obtained from n-alkanewater/methanol liquid-liquid partitioning systems and from analogous bonded n-alkyl reverse-phase chromatographic systems was used to characterize the sorptive behavior of the bonded phase. The magnitude of an α as a function of bonded alkyl chain length in the chromatographic system suggested a liquid-like partitioning of hydrocarbonaceous solutes into the bonded alkanes. The quasi-solution behavior of the bonded phase is dependent on solute size and bonded chain length. It was also found that liquid-liquid partition coefficients could be obtained using entrained n-alkanes on the corresponding n-alkyl bonded phase chromatographic columns.