RADIOENZYMATIC MICROMETHOD FOR THE DETERMINATION OF UDP-GLUCURONIC ACID
- 1 January 1979
- journal article
- research article
- Vol. 17 (6) , 363-367
Abstract
A new sensitive and specific micromethod for determination of UDP-glucuronic acid is described. Extracts from 2.5 mg of liver are incubated with twice-washed guinea pig microsomes (as a source of UDP-glucuronyl transferase) and [14C]p-nitrophenol. The content of UDP-glucuronic acid is calculated from the [14C]p-nitrophenyl glucuronide/[14C]p-nitrophenol radioactivity ratio and the known amount of introduced [14C]p-nitrophenol. These values are corrected for losses of UDP-glucuronic acid by a calibration experiment, containing a known amount of UDP-glucuronic acid. Mean concentration of UDP-glucuronic acid in mice liver was 171 .mu.mol/kg wet weight.This publication has 4 references indexed in Scilit:
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- Measurement of nanogram quantities of UDP-glucuronic acid in tissuesAnalytical Biochemistry, 1977
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- Nucleotide Activation of Liver Microsomal GlucuronidationJournal of Biological Chemistry, 1961