Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum β-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay
Open Access
- 1 October 2004
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 48 (10) , 4059-4062
- https://doi.org/10.1128/aac.48.10.4059-4062.2004
Abstract
The LightCycler was compared to nested PCR for the detection of bla GES/IBC genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a bla GES/IBC gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.Keywords
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