Production and Release of Acetylcholinesterase by a Primary Cell Culture of Bovine Adrenal Medullary Chromaffin Cells

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Abstract
Bovine adranal chromaffin cells were isolated and maintained as primary culture monolayers. Total acetylcholinesterase (AChE) activity in the cells increased during the culture period and AChE activity appeared in the culture medium. The role of the AChE synthesized by the cells on ACh-evoked release of catecholamine from the cells was examined. A progressive decrease in the efficacy of ACh (5 .times. 10-5 M) to evoke release of [3H]norepinephrine from day 3-15 cultures implies that exogenously applied ACh is hydrolyzed by the nascent AChE synthesized by the cells. Chromaffin cells apparently produce AChE and release it into their immediate environment.
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