In situ identification of idiotype‐positive cells participating in the immune response to phosphorylcholine

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Abstract
The phosphorylcholine idiotype (Id)/anti‐Id system has been used to study the role of antigen‐specific cells in antigen‐induced microenvironmental changes. Anti‐Id staining of lymph nodes following PC immunization shows the presence of Id on follicular dendritic cells at 12 h and in plasma cells beginning at day 3. Germinal centers began to form at day 3, peaking in size and number at days 8‐10. Scattered Id‐positive small lymphocytes are present in germinal centers but with rare exceptions over 98% of germinal center cells are Id‐negative. Idiotype‐positive small lymphocytes are depleted from primary follicles adjacent to germinal centers but not from distant, unstimulated nodes.These results extend previous studies showing architectural alterations in lymph nodes following antigenic stimulation and demonstrate antigen‐specific cells are a prominent component of these antigen‐induced microenvironmental changes.

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