Role of Atrial Natriuretic Factor in Renin Release*

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Abstract
Atrial natriuretic factor (ANF) was studied for its effect on renin release by rat renal cortical slices. ANF (rat) alone (10−9-l0−6M) had no effect on basal renin release, but significantly (P < 0.001) potentiated angiotensin II (All) inhibition of renin secretion in doses as low as 10−9 M. ANF also potentiated the inhibitory effect of All on 8-(N,N-diethylamino) octyl 3,4,5-trimethoxybenzoate hydrochloride (which alters intracellular calcium) and calmidazolium (a calmodulin blocker) effects on renin relase. ANF did not inhibit the action of isoproterenol, which probably acts through cAMP, at doses below micromolar concentrations. Large, probably pharmacological amounts (>10−6 M) produce some inhibition. Since All action on renin is associated with increases in intracellular calcium, our studies suggest that ANF acts by altering the intracellular calcium-calmodulin-mediated steps of All action on this tissue and not via cAMP. (Endocrinology119: 318–322, 1986)