Ca2+ is essential for multistep activation of the heat shock factor in permeabilized cells.
Open Access
- 1 June 1991
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 11 (6) , 3365-3368
- https://doi.org/10.1128/mcb.11.6.3365
Abstract
We have developed a novel permeabilized-cell system to study transcription mechanisms. In permeabilized cells, heat-induced activation of the heat shock factor and transcription of the hsp70 gene require Ca2+. Activation involves at least two steps: Ca(2+)- and heat-dependent activation of heat shock factor binding and a second step, prior to transcription of hsp70, that requires ATP and is sensitive to genistein, a protein kinase inhibitor.Keywords
This publication has 21 references indexed in Scilit:
- Yeast heat shock factor contains separable transient and sustained response transcriptional activatorsCell, 1990
- PDGF-Induced Activation of Phospholipase C Is Not Required for Induction of DNA SynthesisScience, 1990
- Characterization and sequence of a mouse hsp70 gene and its expression in mouse cell linesGene, 1990
- THE HEAT-SHOCK PROTEINSAnnual Review of Genetics, 1988
- Activation in vitro of sequence-specific DNA binding by a human regulatory factorNature, 1988
- Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylationCell, 1988
- Developmental regulation of a constitutively expressed mouse mRNA encoding a 72-kDa heat shock-like proteinDevelopmental Biology, 1988
- Mechanisms of Heat-Shock Gene Activation in Higher EukaryotesAdvances in Genetics, 1987
- Rapid increases in inositol trisphosphate and intracellular Ca++ after heat shockBiochemical and Biophysical Research Communications, 1986
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979