Effects of immunosuppressive drugs on secondary antibody response in vitro

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Abstract
The inhibitory effects of 6-mercaptopurine, Imuran, and chloramphenicol on the in vitro incorporation of radioactive amino acid into antibody have been investigated. Inhibition of antibody synthesis by these drugs was compared to inhibition of protein synthesis within the same tissue cultures. The relative inhibition of antibody synthesis compared to that of protein synthesis provided an index which indicated the specificity and toxicity of the immunosuppressive agent. Imuran, at concentrations of 10–20 μg/ml inhibited antibody synthesis primarily, while at higher concentrations, protein synthesis was similarly affected. 6-Mercaptopurine at 177–250 μg/ml inhibited antibody production to a certain degree, while chloramphenicol at 300 μg/ml showed no preferential inhibition of antibody synthesis.