Immunoelectron Microscopic Localization of Rous Sarcoma Virus Structural Protein p27 in Infected Chicken Embryo Fibroblasts

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Abstract
The immunoperoxidase technique was used to localize, at the ultrastructural level, protein Pr76 (a precursor of the gag gene protein of avian leukemia and sarcoma viruses) in chick embryo fibroblast cells infected with RSV-RAV-2 [Rous sarcoma virus-Rous associated virus type 2) virus. The protein was confined to free and membrane-bound ribosomes. This was often particularly conspicuous in the perinuclear region. No staining was found in the ergastoplasmic cisternae, the Golgi apparatus or the nucleus. This pattern of localization is compatible with previous studies where the protein was searched for in various cell subfractions.