Stable Expression of the cDNA Encoding the Feline CD8.ALPHA. Gene.
- 1 January 1994
- journal article
- Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science
- Vol. 56 (5) , 1001-1003
- https://doi.org/10.1292/jvms.56.1001
Abstract
The stable expression of the alpha chain of the feline cytotoxic T cell differentiation antigen (fCD8 alpha) on Crandell feline kidney cells (CRFK) was carried out by using an expression vector which contains the Rous sarcoma virus long terminal repeat and a neo resistant gene. After three rounds of cloning under G418 selection for over two months, the expression of the feline polypeptide was detected by human monoclonal antibody OKT8.Keywords
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