Characterization, cloning and sequencing of a thermostable endo-(1,3?1,4) ?-glucanase-encoding gene from an alkalophilic Bacillus brevis
- 1 January 1993
- journal article
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 38 (4) , 507-513
- https://doi.org/10.1007/bf00242946
Abstract
A Bacillus brevis gene coding for an endo-(1,3–1,4)-β-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the β-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1,3–1,4)-β-glucanases. The optimum temperature and pH for enzyme activity were 65–70°C and 8–10, respectively. When held at 75°C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.Keywords
This publication has 0 references indexed in Scilit: