Insertion of the full‐length calcium channel α1S subunit into triads of skeletal muscle in vitro
- 23 May 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 474 (1) , 93-98
- https://doi.org/10.1016/s0014-5793(00)01583-0
Abstract
A full-length and a C-terminally truncated form of the calcium channel α1S subunit can be isolated from skeletal muscle. Here we studied whether full-length α1S is functionally incorporated into the skeletal muscle excitation-contraction coupling apparatus. A fusion protein of α1S with the green fluorescent protein attached to its C-terminus (α1S-GFP) or α1S and GFP separately (α1S+GFP) were expressed in dysgenic myotubes, which lack endogenous α1S. Full-length α1S-GFP was targeted into triad junctions and restored calcium currents and excitation-contraction coupling. GFP remained colocalized with α1S, indicating that intact α1S-GFP was inserted into triads and that the C-terminus remained associated with the excitation-contraction coupling apparatusKeywords
This publication has 30 references indexed in Scilit:
- Differential Effects of Ca2+ Channel β1a and β2a Subunits on Complex Formation with α1S and on Current Expression in tsA201 CellsJournal of Biological Chemistry, 1998
- Identification of PK-A Phosphorylation Sites in the Carboxyl Terminus of L-Type Calcium Channel α1 SubunitsBiochemistry, 1996
- The role of Ca2+ ions in excitation-contraction coupling of skeletal muscle fibresBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1995
- Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro.The Journal of cell biology, 1993
- Structure and Functional Expression of an ω-Conotoxin-Sensitive Human N-Type Calcium ChannelScience, 1992
- Structure and functional expression of α1, α2, and β subunits of a novel human neuronal calcium channel subtypeNeuron, 1992
- Molecular cloning of multiple subtypes of a novel rat brain isoform of the α1 subunit of the voltage-dependent calcium channelNeuron, 1991
- Distinct calcium channels are generated by alternative splicing and are differentially expressed in the mammalian CNSNeuron, 1991
- Localization of the α1 and α2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triadsNeuron, 1990
- Subcellular distribution of the 1,4-dihydropyridine receptor in rabbit skeletal muscle in situ: an immunofluorescence and immunocolloidal gold-labeling study.The Journal of cell biology, 1989