A batch reactor for monitoring process dynamics during biodegradation of volatile organics

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Abstract
Data are presented that demonstrate a batch reactor that allows accurate monitoring of process dynamics during biodegradation of volatile organics. Using this system, many samples can be removed in a aseptic fashion, allowing frequent measurements of the concentrations of biomass, electron donors, electron acceptors, and volatile compounds. Additionally, the reactor is well instrumented, allowing the continuous monitoring of pH, oxidation/reduction potential, pressure, and temperature. The reactor is constructed entirely of materials which have minimal interaction with volatile organic compounds. It is also demonstrated that sample removal does not cause losses of volatile compounds since the sampling ports are double sealed, making use of both gastight stainless steel ball valves and Teflon‐lined septa.