Antigen‐antibody complexes suppress antibody production by mouse plasmacytoma cells in vitro

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Abstract
Preformed complexes of 2,4-dinitrophenylated keyhole limpet hemocyanin (DNP-KLH) and anti-DNP or anti-KLH antibodies selectively, suppressed the production of anti-DNP IgA by the plasmacytoma cell line, MOPC 315, in vitro. Complexes made with DNP8.5KLH (8.5 moles DNP/105 daltons carrier) inhibited the secretion of IgA but not its synthesis, whereas complexes prepared with the more highly substituted DNP20KLH inhibited synthesis as well. Moreover, complexes of DNP-KLH and anti-DNP F(ab′)2 fragments were as suppressive as those made with IgG antibodies, suggesting that the mode of presentation of antigenic determinants and not surface Fc receptors may play a major role in this phenomenon.