Characterization and function of pig intestinal sucrase‐isomaltase and its separate subunits
Open Access
- 31 August 1984
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 143 (3) , 575-582
- https://doi.org/10.1111/j.1432-1033.1984.tb08408.x
Abstract
1. Papain-solubilized pig intestinal sucrase-isomaltase was purified to homogeneity in a four-step process with a yield of 50%. 2. The substrate specificities of the two enzyme activities were studied together and separately after inactivation or inhibition of one of the activities. 3. Michaelis constants, maximum velocities and time courses of hydrolysis of several substrates, in particular α-limit dextrins, were used to characterize this complex of α-glucosidases. 4. The participation of the enzyme complex in the hydrolysis of α-limit dextrins and more generally in pathways for starch breakdown in the pig digestive tract is examined and discussed.This publication has 38 references indexed in Scilit:
- A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of d-glucose and choline transport systemsPublished by Elsevier ,2002
- Purification and Characterization of a Pig Intestinal α‐Limit DextrinaseEuropean Journal of Biochemistry, 1983
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Purification of the human intestinal brush border membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973
- Studies on the organization of the brush border in intestinal epithelial cells: V. Subfractionation of enzymatic activities of the microvillus membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1968
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- DISC ELECTROPHORESIS‐I BACKGROUND AND THEORY*Annals of the New York Academy of Sciences, 1964
- Solubilization of the human intestinal disaccharidasesBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1963
- Characterization of Hog Intestinal Invertase as a Glucosido-Invertase. III. Specificity of Purified Invertase.Acta Chemica Scandinavica, 1960