Abstract
Gases released from two decay systems (leaf litter, animal carrion) were analyzed with a simple gas chromatograph. The apparatus provides a convenient method for assessing any metabolic activity that may not be expressed in terms of a release of CO2, the index of activity most commonly used. The chromatograph was further employed in respirometric studies of animals in the laboratory. Techniques are discussed and the system compared with more conventional laboratory respirometers. Respirometric measurements are sensitive and consistent, and results are compatible with those derived from other respirometers. The chromatograph has 3 main advantages over conventional techniques: C metabolism not resulting in the release of CO2 may be measured, O2 consumption and CO2 production are measured independently and simultaneously, and estimation of respiratory rate and respiratory quotient is extremely rapid, minimizing stress of animals studied. Gas chromatography is presented as a technique potentially of great value to ecologists in a variety of fields.