Diverse Roles for Histone H2A Modifications in DNA Damage Response Pathways in Yeast
- 1 May 2007
- journal article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 176 (1) , 15-25
- https://doi.org/10.1534/genetics.106.063792
Abstract
There are many types of DNA damage that are repaired by a multiplicity of different repair pathways. All damage and repair occur in the context of chromatin, and histone modifications are involved in many repair processes. We have analyzed the roles of H2A and its modifications in repair by mutagenizing modifiable residues in the N- and C-terminal tails of yeast H2A and by testing strains containing these mutations in multiple DNA repair assays. We show that residues in both tails are important for homologous recombination and nonhomologous end-joining pathways of double-strand break repair, as well as for survival of UV irradiation and oxidative damage. We show that H2A serine 122 is important for repair and/or survival in each of these assays. We also observe a complex pattern of H2A phosphorylation at residues S122, T126, and S129 in response to different damage conditions. We find that overlapping but nonidentical groups of H2A residues in both tails are involved in different pathways of repair. These data suggest the presence of a set of H2A “damage codes” in which distinct patterns of modifications on both tails of H2A may be used to identify specific types of damage or to promote specific repair pathways.Keywords
This publication has 52 references indexed in Scilit:
- Regulation of NuA4 Histone Acetyltransferase Activity in Transcription and DNA Repair by Phosphorylation of Histone H4Molecular and Cellular Biology, 2005
- Saccharomyces cerevisiae Histone H2A Ser122 Facilitates DNA RepairGenetics, 2005
- Non-homologous end-joining factors of Saccharomyces cerevisiaeFEMS Microbiology Reviews, 2004
- Distribution and Dynamics of Chromatin Modification Induced by a Defined DNA Double-Strand BreakCurrent Biology, 2004
- Role of histone H2A ubiquitination in Polycomb silencingNature, 2004
- Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryoGenes & Development, 2004
- Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaksNature Cell Biology, 2003
- NuA4 Subunit Yng2 Function in Intra-S-Phase DNA Damage ResponseMolecular and Cellular Biology, 2002
- Acetylation of histone H4 by Esa1 is required for DNA double-strand break repairNature, 2002
- Lucky breaks: analysis of recombination in SaccharomycesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2000