Demonstration of cross-reactive antigens in F38 and related mycoplasmas by enzyme-linked immunosorbent assay (ELISA) and immunoblotting

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Abstract
SUMMARY: The ELISA and an immunoblotting technique were used to study F38-type mycoplasmas – an important cause of contagious caprine pleuropneumonia – and a number of related mycoplasma species, subspecies, types or serogroups.Two-way ELISA cross-reactivity was demonstrated between five mycoplasmas, namely strain F38,Mycoplasma mycoidessubsp.mycoides(LC strain),M. equigenilalium, M. primatumand bovine serogroup 7. In addition one-way cross-reactivity was demonstrated between F38 and each of the following mycoplasmas:M. mycoidessubsp.mycoides(two SC strains),M. mycoidessubsp.capri, and bovine serogroup L. F38 andM. capricolumdid not cross-react.Immunoblot analysis, unlike ELISA, revealed that F38 andM. capricolumwere closely related. At least four major protein antigens were shared between F38,M. mycoidessubsp.mycoides(SC and LC strains),M. mycoidessubsp.capriand bovine serogroup 7. The ELISA cross-reactions (above) shown byM. equigenitaliumandM. primatumwith each other, with F38 and with other mycoplasmas were not apparent by immunoblotting.