Effects of preillumination with light of different wavelengths on subsequent dark CO2-fixation in Chlorella cells

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Abstract
The effects of preillumination with monochromatic red or blue light on the subsequent dark 14CO2-fixation in Chlorella cells were studied under aerobic as well as anaerobic conditions. When the cell suspension was made aerobic by bubbling air (CO2-free) throughout the periods of preillumination and the following dark 14CO2-fixation, the initial fixation product was mainly PGA. The radioactive carbon first incorporated in PGA was transferred mostly to aspartate during the later periods of dark 14CO2-fixation. The rate of 14C-incorporation into aspartate after preillumination with blue light was 2 to 3 times as high as that observed after red-light pretreatment. The observations support our previous inference that the activity of PEP carboxylase in Chlorella cells is stimulated by preillumination with blue light. When nitrogen gas was used during preillumination and the subsequent dark fixation, the radioactivity of 14C incorporated during the initial enhanced 14CO2-fixation was eventually transferred to alanine and lactate. The increase in radioactivity of alanine and lactate was more pronounced during dark fixation after preillumination with red light than after preillumination with blue light.

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