Immunohistochemical localization of Y hapten and the structurally related H type‐2 blood‐group antigen on large‐bowel tumours and normal adult tissues

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Abstract
The monoclonal antibody C14/1/46/10 which defines the Y hapten carbohydrate antigen (Brown et a., 1983) was shown, by the indirect immunoperoxidase technique, to stain tissue sections of 96% of colo‐rectal adenocar‐cinomas and 100% of colo‐rectal adenomas. In contrast, a monoclonal antibody defining the related H type‐2 blood‐group antigen was found to stain 86% of adenocarcinomas and 32% of adenomas. In adenocarcinomas the percentage of cells stained for both antigens was, on average, greater in group O than in group A patients. In adenomas a focal pattern of expression was observed, for both Y hapten and H type‐2 antigen, that was characteristic of group A but not group O adenocarcinomas. In the normal mucosa of the distal colon, whilst H type‐2 antigen was absent, Y hapten was found in the immature cells of the crypt bases. A comparison of the cellular phenotypes present in adenocarcinomas, adenomas and normal mucosa of the distal colon supports the adenoma‐carcinoma sequence hypothesis for the genesis of colo‐rectal carcinomas. In normal adult tissues Y hapten was predominantly found on epithelial cells whilst nervous tissues, muscles and connective tissues were negative. The expression of Y hapten in several positive tissues was dependent upon secretor status. A comparison of the expression of Y hapten and H type 2 antigen on normal adult tissues revealed that all of the permutations of expression of these two structually related antigens were possible.

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