Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura‐2
- 8 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 317 (1-2) , 131-134
- https://doi.org/10.1016/0014-5793(93)81507-v
Abstract
A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.Keywords
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