A Simple, Rapid Staining Procedure for Methacrylate Embedded Tissue Sections Using Chromotrope 2R and Methylene Blue

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Abstract
Sections of tissue embedded in glycol methacrylate can be stained in rapid sequence with solutions of 1% aqueous chromotrope 2R adjusted to pH 3 and 0.1% methylene blue to produce sufficient contrast and cellular detail to permit quick visual inspection and/or photomicrography. Solutions of these stains are simple to prepare and are stable over long periods. Staining of sections may be accomplished within six minutes.