Characterization of a New Putative Colonization Factor (CS17) from a Human Enterotoxigenic Escherichia coli of Serotype 0114:821 which Produces Only Heat-Labile Enterotoxin

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Abstract
Enterotoxigenic Escherichia coli (ETEC) of serotype O114:H21, which produced only heat-labile enterotoxin (LT), gave mannose-resistant hemagglutination (MRHA) with bovine erythrocytes. One strain, m0738A, wasshown to possess fimbriae of ∼7.5 nm diameter. On SOS-PAGE two possible fimbrial polypeptides of molecular masses 17.5and 15.5kDa were seen; the 17.5-kDa band was the most prominent. Loss of LT and MRHA together from strain E20738A was associated with loss of a l00-MDa plasmid. An absorbed anti-strain E20738A serum reacted specifically with the 17.5-and 15.5-kDa polypeptides and bound to the intact fimbriae. This antiserum reacted positively in an ELISA with LT-positive E. coli strains of serogroups O8, O15, O48, O114, and O146. The antiserum did not react with ETEC carrying known colonization factors. The term coli-surface-associated antigen (CS) 17 has been used to describe the fimbriae.

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