MLH1, PMS1, and MSH2 Interactions During the Initiation of DNA Mismatch Repair in Yeast
- 19 August 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 265 (5175) , 1091-1093
- https://doi.org/10.1126/science.8066446
Abstract
The discovery that mutations in DNA mismatch repair genes can cause hereditary nonpolyposis colorectal cancer has stimulated interest in understanding the mechanism of DNA mismatch repair in eukaryotes. In the yeast Saccharomyces cerevisiae, DNA mismatch repair requires the MSH2, MLH1, and PMS1 proteins. Experiments revealed that the yeast MLH1 and PMS1 proteins physically associate, possibly forming a heterodimer, and that MLH1 and PMS1 act in concert to bind a MSH2-heteroduplex complex containing a G-T mismatch. Thus, MSH2, MLH1, and PMS1 are likely to form a ternary complex during the initiation of eukaryotic DNA mismatch repair.Keywords
This publication has 30 references indexed in Scilit:
- Mutation of a mutL Homolog in Hereditary Colon CancerScience, 1994
- Mutation in the DNA mismatch repair gene homologue hMLH 1 is associated with hereditary non-polyposis colon cancerNature, 1994
- Protein-DNA Recognition: New Perspectives and Underlying ThemesScience, 1994
- Instability of short tandem repeats (microsatellites) in human cancersNature Genetics, 1994
- Genetic mapping of a second locus predisposing to hereditary non–polyposis colon cancerNature Genetics, 1993
- Clues to the Pathogenesis of Familial Colorectal CancerScience, 1993
- Molecular MatchmakersScience, 1993
- MECHANISMS AND BIOLOGICAL EFFECTS OF MISMATCH REPAIRAnnual Review of Genetics, 1991
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Primary structure of the immI immunity region of bacteriophage P22Journal of Molecular Biology, 1983