Purification of Hepatitis B Surface Antigen Using Polyethylene Glycol, Pepsin and Tween 801
- 1 December 1976
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 31 (s1) , 84-95
- https://doi.org/10.1111/j.1423-0410.1976.tb02226.x
Abstract
A simple, inexpensive procedure for the isolation and purification of HBSAg [hepatitis B surface antigen] from plasma is described. The technique included precipitation of HB/SAg with polyethylene glycol and elimination of normal plasma proteins by digestion with pepsin. Tween 80 was used to remove contaminating lipoprotein(s). This technique resulted in an .apprx. 200-fold gain in the specific activity of HBSAg and yielded .apprx. 20-40% recovery. Rabbits immunized with the purified antigen produced type-specific antibodies to HBSAg without detectable reactivity to normal human plasma antigens.This publication has 24 references indexed in Scilit:
- Australia Antigen ‘ad’ and ‘ay’ Subtypes. Purification and Partial CharacterizationVox Sanguinis, 1975
- Isolation and Purification of Hepatitis B Antigen by ElectrochrornatographyThe Journal of Infectious Diseases, 1975
- Separation of Hepatitis-Associated Antigen (HAA) from Human Plasma for the Production of Rabbit Anti-HAAVox Sanguinis, 1974
- Molecular weight determinations of proteins by polyacrylamide gel electrophoresis with sodium dodecyl sulfate in just the sample solutionBiochemical and Biophysical Research Communications, 1974
- DEAE-Cellulose Chromatography: A Method for Dissociation of Soluble Immune Complexes of Hepatitis B AntigenThe Journal of Infectious Diseases, 1974
- Characterization of Hepatitis B Antigen PolypeptidesIntervirology, 1974
- Affinity chromatography of hepatitis B antigen.The Tohoku Journal of Experimental Medicine, 1974
- SEPARATION OF AUSTRALIA ANTIGEN AND SOME BACTERIAL PROTEINS BY ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELSAnnals of the New York Academy of Sciences, 1973
- Purification and Biophysical Characterization of Hepatitis B AntigenJournal of Clinical Investigation, 1973
- Stability of the Lipid and Protein of Hepatitis-Associated (Australia) AntigenThe Journal of Infectious Diseases, 1971