INVITRO CORRELATES OF TRANSFORMATION IN C3H-10T1/2 CLONE 8 MOUSE CELLS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (8) , 2863-2867
Abstract
Various potential in vitro correlates of malignancy were studied in 4 chemically [5-fluorodeoxyuridine, cigarette smoke condensate, bleomycin, 3-methylcholanthrene] transformed C3H/10T1/2 Clone 8 mouse cell lines and were compared with control cells. The degree of tumorigenicity was best predicted by the relative plating efficiencies of the morphologically transformed cells in soft agar. All transformed cells also showed an increase in extracellular fibrinolytic activity which may be an additional marker for transformation. Intracellular fibrimolytic activity and loss of 125I-labeled cell surface protein (MW 250,000) were not correlated with morphological transformation or tumorigenicity in these cells.