Multiple GF‐1 binding sites flank the erythroid specific transcription unit of the human carbonic anhydrase I gene
- 6 November 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 257 (2) , 451-456
- https://doi.org/10.1016/0014-5793(89)81594-7
Abstract
Six potential GF-1 sites which bind an erythroid factor are present in the 5' and 3' regions flanking the erythroid-speciflc transcription unit of the human carbonic anhydrase 1 (HCAI) gene. When two of these sites are placed upstream of a minimal eukaryotic promoter they confer upregulated expression in erythroid over non-erythroid cells. The presence of the erythroid factor in TPA-treated HEL cells in which the level of HCAI transcript has greatly decreased and in non-HCAI-expressing K562 cells suggests that in these cases the presence of the factor is not sufficient for HCAI expression.Keywords
This publication has 31 references indexed in Scilit:
- Carbonic anhydrase II is induced in HL‐60 cells by 1,25‐dihydroxyvitamin D3: A model for osteoclast gene regulationFEBS Letters, 1989
- Regulation of Inducible and Tissue-Specific Gene ExpressionScience, 1987
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- High-sensitivity S1 mapping with single-stranded [32PIDNA probes synthesized from bacteriophage M13mp templatesGene, 1984
- HEL Cells: A New Human Erythroleukemia Cell Line with Spontaneous and Induced Globin ExpressionScience, 1982
- Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factorThe Journal of Experimental Medicine, 1981
- Structure and in Vitro Transcription of Human Globin GenesScience, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Human α-globin gene expression following chromosomal dependent gene transfer into mouse erythroleukemia cellsCell, 1978
- Continuous growth and differentiation of human myeloid leukaemic cells in suspension cultureNature, 1977