Immune Complex of Banana Oxalate Oxidase: Use in Quantitation of Urinary Oxalate
- 1 January 1986
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 19 (19-20) , 1987-1999
- https://doi.org/10.1080/00032718608064541
Abstract
Oxalate oxidase (oxalate:oxygen oxidoreductase, EC 1.2.3.4), isolated from ripe banana peel was precipitated by rat antiserum. Compared with native enzyme, this immune complex possessed higher specific activity, greater stability, broader pH range for optimal activity and higher resistance to thermal and heavy metal inactivation. These improved characteristics of the immune complex make it ideally suited for its use in the determination of urinary oxalate. A simple method of determining oxalate in urine samples, using this preparation is described.This publication has 15 references indexed in Scilit:
- Immobilized enzyme electrode for the determination of oxalate in urineAnalytical Chemistry, 1986
- Degradation of oxalate in rats implanted with immobilized oxalate oxidaseFEBS Letters, 1986
- Comparison of Oxalate Oxidase Enzyme Electrodes for Urinary Oxalate DeterminationsAnalytical Letters, 1986
- Spectrophotometric Determination of Oxalate in Urine and Plasma with Oxalate Oxidase1The Journal of Biochemistry, 1985
- Continuous-Flow Assay for Urinary Oxalate Using Immobilised Oxalate OxidaseAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1985
- Measurement of urinary oxalate in the presence of ascorbic acidClinica Chimica Acta; International Journal of Clinical Chemistry, 1985
- Determination of Oxalate with Immobilized Oxalate Oxidase in an Enzyme ThermistorAnalytical Letters, 1985